disease of cellular proliferation//disease of anatomical entity
Define:
A carcinoma originating in the lung and the most common lung cancer type in never-smokers. Malignant cells exhibit distinct features such as glandular epithelial, or tubular morphology. Mutations in KRAS, EGFR, BRAF, and ERBB2 genes are associated with this cancer.
A lung non-small cell carcinoma that derives_from epithelial cells of glandular origin.
Alias:
Lung Adenocarcinoma
adenocarcinoma of lung//bronchogenic lung adenocarcinoma//nonsmall cell adenocarcinoma [EXACT]
PCR//Western blot//Transfection//Cell apoptosis assay//Cell proliferation assay//Pull down assay//tRF and tiRNA arrays
Description:
We show that tRF-29-79 is downregulated in LUAD and downregulation of tRF-29-79 is associated with poorer prognosis. In vivo and in vitro assay reveal that tRF-29-79 inhibits proliferation, migration and invasion of LUAD cells.
Comparision:
Cancer VS Normal
Mechanism:
we discovered that tRF-29-79 interacts with the RNA-binding protein PTBP1 and facilitates the transportation of PTBP1 from nucleus to cytoplasm, which regulates alternative splicing in the 3' untranslated region (UTR) of SLC1A5 pre-mRNA. Given that SLC1A5 is a core transporter of glutamine, we proved that tRF-29-79 mediate glutamine metabolism of LUAD through affecting the stability of SLC1A5 mRNA, thus exerts its anticancer function. In summary, our findings uncover the novel mechanism that tRF-29-79 participates in glutamine metabolism through interacting with PTBP1 and regulating alternative splicing in the 3' UTR of SLC1A5 pre-mRNA.