| [1] PubMed ID: | 39220268 |
| Disease Name: | Pulmonary Disease, Chronic Obstructive |
| Tissue: | N/A |
| Dysfunction Pattern: | Of note, dimerization of the molecule has been shown to increase its stability,50 potentially contributing to its abundant accumulation in circulation. While the predominant existence of 5′-GlyGCC as a circulating sncRNA aligns with previous studies,51 the sequence detected in our study is longer, specifically a 34-nt molecule of 5′-GlyGCC. We assume that the lack of T4 PNK treatment in sncRNA sequencing could result in the selective detection of secondary cleaved fragments that contain a 3′-OH end. |
| Validated Method: | RT-PCR//Transfection//ELISA//High-throughput sequencing |
| Description: | Circulating RNAs have emerged as novel biomarkers and functional molecules in diverse areas of disease studies. While previous investigations into circulating sncRNAs in the biofluids of COPD patients have primarily focused on miRNAs through the use of standard RNA-seq,25,26,27,28 it is important to note that the majority of extracellular sncRNAs contain either a cP or 3′-P24 and are thus not captured by standard RNA-seq. Moreover, even a single cP-containing tRNA half, rRF, or mRF species can be significantly more abundant than total miRNAs in tissues30 and EVs.24 |
| Comparision: | N/A |
| Mechanism: | N/A |
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