| [1] PubMed ID: | 39220268 |
| Disease Name: | Pulmonary Disease, Chronic Obstructive |
| Tissue: | Blood |
| Dysfunction Pattern: | N/A |
| Validated Method: | RT-qPCR/Transfection/ELISA//High-throughput sequencing |
| Description: | Circulating RNAs have emerged as novel biomarkers and functional molecules in diverse areas of disease studies. While previous investigations into circulating sncRNAs in the biofluids of COPD patients have primarily focused on miRNAs through the use of standard RNA-seq,25,26,27,28 it is important to note that the majority of extracellular sncRNAs contain either a cP or 3′-P24 and are thus not captured by standard RNA-seq. Moreover, even a single cP-containing tRNA half, rRF, or mRF species can be significantly more abundant than total miRNAs in tissues30 and EVs.24 This suggests that standard RNA-seq data contain critical biases by excluding the majority of circulating sncRNAs.21,22,23 In this study, by incorporating pre-treatment of plasma RNA with T6 PNK into the sncRNA sequencing process, we obtained a comprehensive and accurate representation of sncRNAs in human plasma samples and discerned their differential profiles between healthy individuals and COPD patients. |
| Comparision: | Disease VS Control |
| Mechanism: | While we acknowledge certain limitations in the present study including the small sample size and the lack of the detailed clinical information, our studies provide the first sequence information of circulating sncRNAs in COPD patients and the basis for exploring unique species of sncRNAs as biomarkers in COPD diagnosis. These approaches, which can identify circulating sncRNAs that elude standard RNA-seq—such as cP-containing 5′-tRNA halves19,34 that are actually are predominant in circulating tRNA-derived sncRNAs—should be implemented in exRNA research for other diseases to advance our understanding of the molecular landscape and potential biomarkers. It is also important to note that post-transcriptional modifications of RNAs, which may impede reverse transcription, could introduce biases in sequencing results. Exploring potentially underrepresented sncRNAs due to modifications can be achieved by sncRNA sequencing procedures that include a step to remove some of the RT-hindering modifications.47,48,51 |
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