Entry Detail


General Information

Database ID:TRD00101
Confidence:Very High
Contents:>> tsRNA Information
>> tsRNA Association Statistics
>> Disease Information
>> Disease Association Statistics
>> Evidence Support
>> Reference



tsRNA Information

tsRNA Name:tRF-32-87R8WP9N1EWJM
tsRNA Type:5'-tiRNA
Amino acid and Anticodon:GluCTC
Sequence:TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC
Related Target:N/A
Predicted Target:TFCP2L1//EME2//SLC25A48//TMEM92//RFTN1//SLC25A6//PAQR4//CCDC159//PDE12//ALKBH2
External Links:
MINTbase ID:tRF-32-87R8WP9N1EWJM
tRFdb ID:N/A

[1] gtRNAdb_ID:tRNA-Glu-CTC-2-1
Anticodon:GluCTC
tRNA_number:trna59
Chromosome:1
Strand:+
Coordinate:Start Site(bp): 249168447        End Site(bp): 249168478

[2] gtRNAdb_ID:tRNA-Glu-CTC-1-1
Anticodon:GluCTC
tRNA_number:trna116
Chromosome:1
Strand:-
Coordinate:Start Site(bp): 145399273        End Site(bp): 145399304

[3] gtRNAdb_ID:tRNA-Glu-CTC-1-5
Anticodon:GluCTC
tRNA_number:trna71
Chromosome:1
Strand:-
Coordinate:Start Site(bp): 161439229        End Site(bp): 161439260

[4] gtRNAdb_ID:tRNA-Glu-CTC-1-4
Anticodon:GluCTC
tRNA_number:trna74
Chromosome:1
Strand:-
Coordinate:Start Site(bp): 161431849        End Site(bp): 161431880

[5] gtRNAdb_ID:tRNA-Glu-CTC-1-3
Anticodon:GluCTC
tRNA_number:trna77
Chromosome:1
Strand:-
Coordinate:Start Site(bp): 161424438        End Site(bp): 161424469

[6] gtRNAdb_ID:tRNA-Glu-CTC-1-2
Anticodon:GluCTC
tRNA_number:trna80
Chromosome:1
Strand:-
Coordinate:Start Site(bp): 161417058        End Site(bp): 161417089

[7] gtRNAdb_ID:tRNA-Glu-CTC-1-6
Anticodon:GluCTC
tRNA_number:trna77
Chromosome:6
Strand:+
Coordinate:Start Site(bp): 28949976        End Site(bp): 28950007

[8] gtRNAdb_ID:tRNA-Glu-CTC-1-7
Anticodon:GluCTC
tRNA_number:trna87
Chromosome:6
Strand:-
Coordinate:Start Site(bp): 126101433        End Site(bp): 126101464



tsRNA Association Statistics

Total Associated Disease Number:2
More Information
Network:
(Display the first 15 nodes)



Disease Information

 MeSHDisease Ontology
Disease ID:D001249DOID:2841
Disease Name:Asthmaasthma
Category:MeSHDisease Ontology
Type:Respiratory Tract Diseases//Immune System Diseasesdisease of anatomical entity
Define:A form of bronchial disorder with three distinct components: airway hyper-responsiveness (RESPIRATORY HYPERSENSITIVITY), airway INFLAMMATION, and intermittent AIRWAY OBSTRUCTION. It is characterized by spasmodic contraction of airway smooth muscle, WHEEZING, and dyspnea (DYSPNEA, PAROXYSMAL).A bronchial disease that is characterized by chronic inflammation and narrowing of the airways, which is caused by a combination of environmental and genetic factors. The disease has_symptom recurring periods of wheezing (a whistling sound while breathing), has_symptom chest tightness, has_symptom shortness of breath, has_symptom mucus production and has_symptom coughing.
Alias:Asthma, Bronchial//Bronchial Asthmabronchial hyperreactivity//chronic obstructive asthma//chronic obstructive asthma with acute exacerbation//chronic obstructive asthma with status asthmaticus



Disease Association Statistics

Total Associated tsRNA Number:4
More Information
Network:
(Display the first 15 nodes)



Evidence Support

Strong Evidence:RT-PCR//Western blot//Northern blot//Immunoblot//ELISA
Weak Evidence:High-throughput sequencing



Reference

[1] PubMed ID:40442279
Disease Name:Asthma
Tissue:Blood
Dysfunction Pattern:N/A
Validated Method:RT-PCR//Western blot//Northern blot//Immunoblot//ELISA//High-throughput sequencing
Description:Our key findings reveal a significant upregulation of ANG expression in circulating leukocytes of severe asthma patients, alongside notable alterations in the extracellular levels of 5’-tRH Glu and 5’-tRH Gly in both blood and sputum samples from individuals with asthma. These results are further supported by in vitro experiments using allergen-stimulated macrophages, suggesting a conserved mechanism across various cell types. Overall, the present findings identify soluble mediators in both blood and pulmonary secretions that hold promise for applying liquid biopsies in characterizing clinical phenotypes of asthma.
Comparision:Disease VS Control
Mechanism:These molecules were selected based on a bioinformatic analysis conducted in our laboratory, analyzing publicly available sRNA seq data from sputum, saliva, and BAL samples23. The tRNA-derived fraction in these samples mainly comprised tRHs derived from GLU, GLY, LYS, or VAL. These findings align with existing literature describing these fragments as highly abundant in blood and other analyzed biofluids18,40. Among them, we chose 5’-tRH Glu and 5’-tRH Gly due to their documented enhanced stability in extracellular fluids. This stability arises from their capacity to form various secondary structures, acting as stable reservoirs in the extracellular space27,41.